Other closely related tetrazolium dyes including XTT, MTS and the WSTs, are used in conjunction with the intermediate electron acceptor, 1-methoxy phenazine methosulfate (PMS). With WST-1, which is cell-impermeable, reduction occurs outside the cell via plasma membrane electron transport. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic.
The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1. Applications: Non-Radioactive Cell Proliferation or Cytotoxicity Assay. The Cell Counting Kit-8 (CCK8) is a one-bottle kit that requires no premixing. This non-radioactive, sensitive colorimetric assay allows accurate live cell counting in a cell proliferation or cytotoxicity assay.
The XTT Cell Viability Kit detects formazan dye produced from XTT conversion by mitochondrial enzymes in cells. Because these mitochondrial enzymes are inactivated shortly after cell death, the orange colored formazan dye only appears in viable cells. This XTT Cell Viability Kit is expected to work in most cells lines. Variable with cell type and incubation time employed in the assay, 0.2-2x10.
The absorbance method of detection used by the MTT assay and other tetrazolium reduction assays (i.e., MTS, XTT, WST-1, WST-8) is generally less sensitive than fluorescent and luminescent methods for detecting viable cell number. This lack of sensitivity severely limits the ability to miniaturize the assay for HTS applications. Although the detection sensitivity varies widely among cell types.
The MTT assay and the MTS assay are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple color. A main application allows to assess the viability (cell counting) and the proliferation of cells (cell culture assays).
These compounds fall into two basic categories: 1) MTT which is positively charged and readily penetrates viable eukaryotic cells and 2) those such as MTS, XTT, and WST-1 which are negatively charged and do not readily penetrate cells.
Regarding MTS, it is true that the cells are still stable, but I'd like to add that the sensitivity of the MTS assay is far lower than the Cell counting Kit-8 assay which uses WST-8 instead. The sensitivity of the MTS assay is about half that of WST-8 for adherent cells and about one third for leukocytes. Log in or register to post comments.
The kit utilizes a highly water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is directly in proportion to the numbers of living cells. The detection sensitivity by CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
The WST-1 Cell Proliferation Colorimetric Assay Kit provides a fast and sensitive way for quantification of cell proliferation and viability. Cell proliferation causes the increase in the amount of formazan dye formed that can be quantified by measuring the absorbance of the dye solution at 440 nm using microtiter plate reader. The kit can be used for the analysis of cell proliferation in.
The amount of the formazan dye, generated by the activities of dehydrogenases in cells, is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1. Cell Viability Detection Mechanism with CCK-8.
The amount of the formazan dyes generated by the activities of dehydrogenases in cells, are directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1. Highest Sensitivity No Toxicity to Cells.
Several tetrazolium compounds are available as substrates such as MTT, MTS, XTT, and WST-1. Among these compounds, only MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide) is a positively charged compound which can penetrate viable eukaryotic cells and reflect cytosolic metabolic activities.
The MTT and XTT colorimetric bactericidal assays were conducted by incubating antibody-opsonized bacteria with neutrophils in microtiter plates for 30 and 60 min at ratios of ten and 100 bacteria per neutrophil. Neutrophils were then lysed with saponin and samples were incubated 30 min with MTT or XTT plus coenzyme Q (CQ).
The CyQUANT XTT Cell Viability Assay is a complete and optimized kit for the detection of mammalian cell viability. The assay kit includes XTT Reagent (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) and Electron Coupling Reagent. The XTT reagent, which is a tetrazolium-based compound, is sensitive to cellular redox potential and in the presence of actively respiring.
WST-1 assay is much like MTT assay and the MTS assay, they are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving an orange yellow. A main application allows assessing the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential.
MTT Assay Kit ab211091 is an easy-to-use, non-radioactive, and high-throughput assay for measuring cell proliferation, cell viability and cytotoxicity. The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product.
The amount of formazan product is directly proportional to the enzymatic activity of dehydrogenases and the number of living cells. With its high sensitivity and low cytotoxicity, Cyto-X outperforms traditional assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
WST-1 Cell Proliferation and Cytotoxicity Assay Kit is a sensitive and accurate assay for cell cytotoxicity and proliferation. WST-1 assay is similar to MTT assay and MTS assay, they are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS,and WSTs) to formazan dyes, giving a purple yellow color. A main application allows assessing the viability.
However, the second generation tetrazolium dyes (XTT, WST-1 and to some extent, MTS) that form water-soluble formazans and require an intermediate electron acceptor for reduction, are characterised by a net negative charge and therefore are largely cell-impermeable.